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gel filtration chromatography  (TaKaRa)


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    Structured Review

    TaKaRa gel filtration chromatography
    Gel Filtration Chromatography, supplied by TaKaRa, used in various techniques. Bioz Stars score: 94/100, based on 74 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/chroma+spin+200+column+chromatography/CHROMA+SPIN+%2BTE-200+Columns/pm17471289-66-39-45
    Average 94 stars, based on 74 article reviews
    gel filtration chromatography - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Purification:

    Article Title: Gene expression profile of long-lived snell dwarf mice.
    Article Snippet: .. The purification of the labeled cDNA from unincorporated 32 P-labeled nucleotides was achieved with CHROMA SPIN-200 column chromatography (Clontech, Palo Alto, CA). cDNA-fractions of highest activity were pooled and hybridized to the Mouse Atlas 1.2 Array and 1.2 Array II membranes, each of which contains 1176 spotted mouse cDNA fragments as well as control spots. .. After prehybridization (30 minutes at 68 C in ExpressHyb; Clontech) supplemented with 100 g/ml of sheared salmon testes DNA (Sigma), the heat-denatured cDNA target preparation was added.

    Article Title: Upregulation of Tyrosine Kinase TKT by the Epstein-Barr Virus Transactivator Zta
    Article Snippet: .. The mixtures were then purified by phenol-chloroform extraction and CHROMA SPIN-200 column chromatography (Clontech, Palo Alto, Calif.). ..

    Article Title: Comparison of gene expression of 2-mo denervated, 2-mo stimulated-denervated, and control rat skeletal muscles.
    Article Snippet: Kostrominova, Tatiana Y., Douglas E. Dow, Robert G. Dennis, Richard A. Miller, and John A. Faulkner.. Comparison of gene expression of 2-mo denervated, 2-mo stimulated-denervated, and control rat skeletal muscles.. Physiol Genomics 22: 227–243, 2005.

    Labeling:

    Article Title: Gene expression profile of long-lived snell dwarf mice.
    Article Snippet: .. The purification of the labeled cDNA from unincorporated 32 P-labeled nucleotides was achieved with CHROMA SPIN-200 column chromatography (Clontech, Palo Alto, CA). cDNA-fractions of highest activity were pooled and hybridized to the Mouse Atlas 1.2 Array and 1.2 Array II membranes, each of which contains 1176 spotted mouse cDNA fragments as well as control spots. .. After prehybridization (30 minutes at 68 C in ExpressHyb; Clontech) supplemented with 100 g/ml of sheared salmon testes DNA (Sigma), the heat-denatured cDNA target preparation was added.

    Article Title: Comparison of gene expression of 2-mo denervated, 2-mo stimulated-denervated, and control rat skeletal muscles.
    Article Snippet: Kostrominova, Tatiana Y., Douglas E. Dow, Robert G. Dennis, Richard A. Miller, and John A. Faulkner.. Comparison of gene expression of 2-mo denervated, 2-mo stimulated-denervated, and control rat skeletal muscles.. Physiol Genomics 22: 227–243, 2005.

    Column Chromatography:

    Article Title: Gene expression profile of long-lived snell dwarf mice.
    Article Snippet: .. The purification of the labeled cDNA from unincorporated 32 P-labeled nucleotides was achieved with CHROMA SPIN-200 column chromatography (Clontech, Palo Alto, CA). cDNA-fractions of highest activity were pooled and hybridized to the Mouse Atlas 1.2 Array and 1.2 Array II membranes, each of which contains 1176 spotted mouse cDNA fragments as well as control spots. .. After prehybridization (30 minutes at 68 C in ExpressHyb; Clontech) supplemented with 100 g/ml of sheared salmon testes DNA (Sigma), the heat-denatured cDNA target preparation was added.

    Article Title: A combined approach for gene discovery identifies insulin-like growth factor-binding protein-related protein 1 as a new gene implicated in human endometrial receptivity.
    Article Snippet: .. Unincorporated 32P-labeled nucleotides were removed by CHROMA SPIN-200 column chromatography (CLONTECH Laboratories, Inc.). .. After prehybridization for 30 min at 68 C in Express Hyb solution (CLONTECH Laboratories, Inc.), the probes were hybridized overnight with the cDNA array filters.

    Article Title: Upregulation of Tyrosine Kinase TKT by the Epstein-Barr Virus Transactivator Zta
    Article Snippet: .. The mixtures were then purified by phenol-chloroform extraction and CHROMA SPIN-200 column chromatography (Clontech, Palo Alto, Calif.). ..

    Article Title: Comparison of gene expression of 2-mo denervated, 2-mo stimulated-denervated, and control rat skeletal muscles.
    Article Snippet: Kostrominova, Tatiana Y., Douglas E. Dow, Robert G. Dennis, Richard A. Miller, and John A. Faulkner.. Comparison of gene expression of 2-mo denervated, 2-mo stimulated-denervated, and control rat skeletal muscles.. Physiol Genomics 22: 227–243, 2005.

    Article Title: Expression of interleukin-15 in human endometrium and decidua.
    Article Snippet: Briefly, total RNA (20 μg) of human endometrium from the proliferative and from the secretory phases were treated with 5 μl of DNase I (Boehringer Mannheim) at 37°C for 1 h. RNA was then precipitated at –80°C for 30 min using 0.1 volume of 3 mol/l sodium acetate pH 5.2 and 2.5 volume of 100% ethanol and converted into 32P-labelled first strand cDNA by means of Superscript II reverse transcriptase (Gibco BRL). .. Unincorporated 32P-labelled nucleotides were removed by CHROMA SPIN-200 column chromatography (Clontech). .. After prehybridization [30 min at 68°C in ExpressHyb (Clontech)], the heat-denatured probes were hybridized to Atlas human cDNA expression array membranes for 18 h at 68°C.

    Article Title: Inhibition of the putative tumor suppressor gene TIMP-3 by tumor-derived p53 mutants and wild type p53.
    Article Snippet: Mouse Atlas cDNA Expression Arrays (Clontech) were prehybridized in 50% formamide, 66SSC, 0.1% SDS, 1 mM EDTA, 26Denhardt's, and 100 mg/ml sheared single stranded DNA at 428C for 24 h. One microgram each of poly(A)+ mRNA from the 10(3), CM248.2 and MMTV32 cell lines were reverse transcribed in the presence [a-32P]-dCTP to synthesize labeled cDNA. .. The cDNA was puri®ed by CHROMA SPIN-200 column chromatography (Clontech). ..

    Activity Assay:

    Article Title: Gene expression profile of long-lived snell dwarf mice.
    Article Snippet: .. The purification of the labeled cDNA from unincorporated 32 P-labeled nucleotides was achieved with CHROMA SPIN-200 column chromatography (Clontech, Palo Alto, CA). cDNA-fractions of highest activity were pooled and hybridized to the Mouse Atlas 1.2 Array and 1.2 Array II membranes, each of which contains 1176 spotted mouse cDNA fragments as well as control spots. .. After prehybridization (30 minutes at 68 C in ExpressHyb; Clontech) supplemented with 100 g/ml of sheared salmon testes DNA (Sigma), the heat-denatured cDNA target preparation was added.

    Control:

    Article Title: Gene expression profile of long-lived snell dwarf mice.
    Article Snippet: .. The purification of the labeled cDNA from unincorporated 32 P-labeled nucleotides was achieved with CHROMA SPIN-200 column chromatography (Clontech, Palo Alto, CA). cDNA-fractions of highest activity were pooled and hybridized to the Mouse Atlas 1.2 Array and 1.2 Array II membranes, each of which contains 1176 spotted mouse cDNA fragments as well as control spots. .. After prehybridization (30 minutes at 68 C in ExpressHyb; Clontech) supplemented with 100 g/ml of sheared salmon testes DNA (Sigma), the heat-denatured cDNA target preparation was added.

    Extraction:

    Article Title: Upregulation of Tyrosine Kinase TKT by the Epstein-Barr Virus Transactivator Zta
    Article Snippet: .. The mixtures were then purified by phenol-chloroform extraction and CHROMA SPIN-200 column chromatography (Clontech, Palo Alto, Calif.). ..

    Radioactivity:

    Article Title: Comparison of gene expression of 2-mo denervated, 2-mo stimulated-denervated, and control rat skeletal muscles.
    Article Snippet: Kostrominova, Tatiana Y., Douglas E. Dow, Robert G. Dennis, Richard A. Miller, and John A. Faulkner.. Comparison of gene expression of 2-mo denervated, 2-mo stimulated-denervated, and control rat skeletal muscles.. Physiol Genomics 22: 227–243, 2005.



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